Key Considerations When Selecting HPLC Reference Standards (RMs)

How to Choose Reference Materials (RMs) for HPLC: Key Selection Criteria

High-performance liquid chromatography (HPLC) is one of the most accurate and widely used analytical methods in chemistry, pharmaceuticals, food industry, and environmental monitoring. However, the reliability of results largely depends on the quality of reference materials (RMs) used.

Our experts recommend paying attention to 5 key parameters when selecting RMs for HPLC.

Documentation

Reference materials must be accompanied by valid documents confirming their status. These should include:

• Certified characteristics;
• Measurement uncertainty;
• Margin of error;
• Certification methodology.

Lack of proper documentation may lead to incorrect analysis results.

Certified Characteristics

It's important to consider both qualitative and quantitative indicators of the RM's certified characteristics. The certified characteristics must precisely match the research criteria. For example, when analyzing the content of a specific substance, the RM should have a confirmed concentration value.

Stability

Expiration date and storage conditions are critically important parameters. Unopened reference materials should be stored under manufacturer-recommended conditions (temperature, humidity, light protection). After opening, many RMs require immediate use unless otherwise specified in the documentation.

Manufacturer

We recommend purchasing RMs only from verified suppliers who:

• Provide complete documentation packages,
• Guarantee quality standard compliance,
• Have positive market reputation.

Why Choose NCSS?

The National Center of Reference Samples offers:

• Wide range of approved-type reference materials (SRMs) used across various sectors of the Russian economy.
• Customized RM selection tailored to customer requirements.
• Guaranteed quality and stability of characteristics in accordance with regulatory requirements.